Mosebetsi oa homogenizer ke ho kopanya lintho tse nang le mefuta e fapaneng ka mokhoa o ts'oanang ka thipa ea eona ea ho kuta ka lebelo le phahameng, e le hore lisebelisoa tse tala li ka kopana hantle, ho finyella boemo bo botle ba emulsification, le ho bapala karolo ea ho felisa li-bubble.

Matla a maholo a homogenizer, lebelo le leholo, le ho sebetsa hantle nakong ea tlhahiso.Ha nako e telele kholomo e ka sehloohong ea homogenizer e ntse e le, matla a homogenizable haholoanyane.

Molao-motheo oa homogenizer o atisang ho sebelisoa laboratoring: kopanya sampole ea liteko le tharollo kapa solvent ka ho lekana ho fihlela tharollo e tloaelehileng e hlokoang ke teko.Homogenizer e ka aroloa ka mekhahlelo e meraro e latelang ho latela mokhoa oa eona oa ho sebetsa:

Ultrasonic homogenizer

Molao-motheo: Molao-motheo oa ho sebelisa leqhubu la molumo le leqhubu la ultrasonic ho hatella le ho hola ka potlako ha u kopana le lintho.Tlas'a ketso ea leqhubu la ultrasonic, ha thepa e le halofo ea potoloho ea ho atolosa, mokelikeli oa lintho tse bonahalang o tla atoloha e le li-bubble tlas'a tsitsipano;Nakong ea halofo ea potoloho ea khatello, li-bubble lia fokotseha.Ha khatello e fetoha haholo 'me khatello e tlaase ho feta khatello e tlaase, li-bubble tse hatelitsoeng li tla oa ka potlako,' me "cavitation" e tla hlaha ka mokelikeli.Ketsahalo ena e tla nyamela ka phetoho ea khatello le ho se leka-lekane ha khatello ea ka ntle.Nakong eo "cavitation" e nyamelang, khatello le mocheso o pota-potileng mokelikeli o tla eketseha haholo, o bapala karolo e rarahaneng haholo le e matla ea mechine e susumetsang, E le ho finyella morero oa homogenization.

Sebaka sa ts'ebeliso: ho sithabela ha lisele tse fapaneng le lysis ea lisele, ho ntšoa ha li-organelles, li-nucleic acid, liprotheine, le emulsification le homogenization ea lisampole tse ling tsa indasteri.

Melemo: E bonolo ho e sebelisa, 'me e khona ho sebetsana le mefuta e fapaneng ea lisampole ka ho fetola li-probes tse fapaneng;Emulsification e ntle le phello ea homogenization, e loketseng ts'ebetso e le 'ngoe ea mohlala.

Mefokolo: disampole tse ngata ha di kgone ho sebetswa ka nako e le nngwe.Mehlala e fapaneng e hloka ho nkeloa sebaka kapa ho hloekisoa, ho eketsa monyetla oa ho silafala lipakeng tsa lisampole;E na le tšusumetso e itseng ho lisampole tsa likokoana-hloko tse nang le litlhoko tse khethehileng.

Etsa lipatlisiso tsa rotary blade homogenizer

Molao-motheo: Mofuta ona o sebelisetsoa ho arola, ho kopanya, ho pshatla le homogenize ka ho potoloha pestle ea ho sila ka homogenizer.E loketse ho sebetsana le disampole ka boima bo matla.

Sebaka sa ts'ebeliso: E ka sebelisoa ho hasanya lisele tsa liphoofolo / limela, ho ntša nucleic acid, protheine, joalo-joalo ka lysate, hape e sebelisoa ho resin ea indasteri le ho etsa li-pigment suspension/emulsion, joalo-joalo.

Melemo: lebelo le tlase, torque e kholo, ha ho lerata, joalo-joalo Ho bonolo ho e sebelisa.Ka ho fetola li-probes tse fapaneng, mefuta e fapaneng ea lisampole e ka sebetsoa.Ho bonolo ho sebetsa mme ho loketse haholoanyane ho sebetsa mohlala o le mong.

Mefokolo: disampole tse ngata ha di kgone ho sebetswa ka nako e le nngwe.Mehlala e fapaneng e hloka ho nkeloa sebaka kapa ho hloekisoa, ho eketsa monyetla oa ho silafala lipakeng tsa lisampole;Li-homogenizers tse joalo ha li nkoe bakeng sa kalafo ea disampole tse teteaneng tse kang libaktheria, tomoso le li-fungus tse ling.

Ho otla homogenizer (hape e bitsoa homogenizer ea ho kokota le ho sila lifaha homogenizer)

Molao-motheo: Tsoela pele ho otla mokotla ka boto ea hamore.Khatello e hlahisoang e ka senya le ho kopanya lisebelisoa tse ka mokotleng.Homogenizer ea lifaha tse silang e sebelisetsoa ho sila le homogenize sampole ka ho beha sampole le lifaha tse tsamaellanang ka har'a tube ea teko, ho potoloha le ho thothomela ka lebelo le phahameng ka litekanyo tse tharo, le ho pshatla sampole ka ho tlanya ka lebelo le phahameng la sefaha se silang.

Sebaka sa ts'ebeliso: E sebelisoa haholo bakeng sa ho pshatla lithane tsa liphoofolo le limela, algae, baktheria, tomoso, li-fungus kapa hlobo, hammoho le li-sporophyte tse fapaneng, le ho ntša DNA/RNA le protheine.

Melemo: E ka sebetsana ka katleho le lisampole tse manganga tse kenyelletsang masapo, li-spores, mobu, joalo-joalo senoelo se seng le se seng sa homogenizer se na le thipa ea homogenizer ho qoba tšilafalo ea sefapano, e leng bonolo le e sebetsang hantle ho sebetsa, 'me ho molemo ho sebetsana le lisampole tse senyehang.

Mefokolo: Ha e khone ho sebetsana le lisampole tse kholo tsa molumo.Bokhoni ba ho sebetsa ba sampole e le 'ngoe hangata bo ka tlase ho 1.5ml, 'me bo hloka ho sebelisoa hammoho le mokotla o tšoanang oa homogeneous, ka hona, tlhahiso ea lisebelisoa le lisebelisoa e phahame.